mouse anti jnk  (Proteintech)

Journal: bioRxiv

Article Title: Direct Binding of FGFR3 Autoantibodies to Sensory Neurons Drives Hyperexcitability and Mechanical Pain Hypersensitivity

doi: 10.1101/2025.06.01.657230

Figure Lengend Snippet: Rats were injected in the paw with α-FGFR3-positive serum (50 µL, diluted 1:10) or IgG depleted serum. DRG were harvested 2h after injection. ( A ) Representative immunoblots depicting levels of ERK, JNK1/2, and p-p38 as well as their respective phosphorylated counterparts. ( B ) Bar graph with scatter plot showing levels of phosphorylated ERK, JNK, and p38 in DRG from rats injected as indicated. Mean ± SEM, *p<0.05, Mann-Whitney test, n=6 rats per group.

Article Snippet: After transfer, the membranes were blocked at room temperature for 1 h with TBST (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1 % Tween 20), 5% (mass/vol) non-fat dry milk, then incubated separately in the primary antibodies FGFR3 (Sigma, Cat#SAB5702794), p38 (Cell Signaling, Cat#8690), pp38 T180/Y182 (Cell Signaling, Cat#4511), JNK1 (Cell Signaling, Cat#3708), pJNK1 T183/Y185 (Cell Signaling, Cat#4668), ERK (Cell Signaling, Cat#4696), pERK T202/Y204 (Cell Signaling, Cat#4370), Actin (Thermo Fisher Scientific, Cat#PA116889) or βIII-Tubulin (Promega, Cat#G7121) in TBST, 5% (mass/vol) BSA, overnight at 4°C.

Techniques: Injection, Western Blot, MANN-WHITNEY